29/11/2021
S***m analysis with the help of at SPU, Qadirabad.
Progressive motility is a vital functional characteristic of ej******ed s***matozoa that governs their ability to pe*****te into, and migrate through, both cervical mucus and the oocyte vestments, and ultimately fertilize the oocyte. A detailed protocol, based on the most common computer-aided s***m analysis (CASA) system with phase contrast microscope optics, is provided for performing reliable assessments of s***m movement pattern characteristics ("kinematics") in semen. The protocol can also be used with washed s***m suspensions where, in addition, the percentages of motile and progressively motile s***matozoa can also be derived. Using CASA technology it is also possible to identify biologically, and hence clinically, important subpopulations of s***matozoa (e.g., those in semen with good mucus-penetrating characteristics, or those showing hyperactivation when incubated under capacitating conditions) by applying multi-parametric definitions on a cell-by-cell basis.
***manalysis
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