03/06/2025
Next up in our Research Spotlight, .palmer showed us the amazing work she’s done in helping to identify the location of two endangered species in Illinois! Here’s what she has to say about her work:
“My work has identified the location of two endangered species, the Salamander Mussel and the Mudpuppy, with the use of environmental DNA, with the goal of directing conservation efforts for the Illinois Department of Natural Resources. As a result of my independent project, live Salamander Mussels were found for the first time in nearly 120 years in Illinois.
Detecting rare species with conventional survey methods has become increasingly difficult, often depleting time and resources. Instead, researchers have developed a novel method for species detection with environmental DNA (eDNA). eDNA is a method by which samples are collected from the target environment, then through DNA extraction and sequence analysis, it can be derived if the species is present or not.
The two species which I observe are the Mudpuppy and the Salamander Mussel. These fully-aquatic species have a unique symbiotic relationship through which the mussel must carry out their larval stage within the external gills of the Mudpuppy. Without Mudpuppies, Salamander Mussels cannot survive. In Illinois, both species populations are declining. The Mudpuppy population, which is more steady, yet state-endangered, is facing decline due to lampricide use and water pollution. Whereas, the mussel is highly endangered in Illinois and has not been visually detected in decades. Both species live under rocks and are most active in winter months.
To determine if eDNA can be used to detect the Mudpuppy and the Salamander Mussel, my methodology consisted of sample collection, filtration, DNA extraction, and qPCR. ”