28/05/2026
This study explored the role of Withania somnifera/Ashwagandha root extract (ARE) on important gut–microbiome parameters in healthy geriatric dogs. The research team hypothesized that ARE might promote a healthy gut by its adaptogenic and anti-inflammatory effects and improve vital parameters for healthy ageing. This randomized, double-blind, placebo-controlled trial was conducted in Telangana, India. Twelve healthy geriatric Beagle dogs aged 12–15 years were enrolled. The dogs were divided into two groups to receive ARE (15 mg/kg, once daily, orally, for two months) or a placebo control. Various parameters were assessed, including serum haematology, biochemical markers, stool parameters, and gut–microbiome parameters. The results showed erythrocyte counts and haemoglobin levels were significantly increased. Moreover, a significant decrease in important serum liver biomarkers (alanine transaminase and aspartate transaminase) was observed in the ARE-treated dogs compared to that in the placebo control group. In addition, the levels of L-citrulline were significantly modulated by ARE intervention, whereas the intervention did not affect intestinal-type alkaline phosphatase, lactate, and carbamoyl-phosphate synthase. Interestingly, the faecal score reduced significantly with ARE, while the faecal pH remained unaltered. Compared to the baseline, ARE significantly decreased two microbial metabolites, propionic acid, and total short chain fatty acid levels after 60 days of intervention, whereas butyrate and acetic acid levels remained unchanged in the faecal samples. In summary, these findings suggest that ARE has gut health promoting benefits in healthy geriatric dogs by improving haematological and biochemical profiles; the levels of L-citrulline; propionic acid; and SCFA; thus, reducing age-related changes by modulating the microbiome and the associated metabolites.
